Working Experience with Solid Phase Antibody Detection Technique
نویسندگان
چکیده
منابع مشابه
Solid-phase fluoroimmunoassay for treponemal antibody.
An objective, solid-phase fluoroimmunoassay for treponemal antibody was developed with a lysate of virulent Treponema pallidum (Nichols strain) adsorbed on cellulose acetate disks. A probe containing both the antigen and control disks is inserted successively into a serum specimen dilution, a buffer rinse, fluoroscein isothiocyanate-conjugated goat anti-human immunoglobulin G, and a second buff...
متن کاملUrinary albumin radioimmunoassay using a solid phase second antibody and solid phase albumin iodination.
The measurement of microalbuminuria in diabetics is now clinically important as a predictor of later overt clinical proteinuria, reflecting advanced diabetic nephropathy [l-3]. Urine albumin assays suitable for routine use in the clinical laboratory are therefore important. Measurement of microalbuminuria by current techniques including immunodiffusion [4] and electroimmunoassay [5] are time-co...
متن کاملSolid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies.
A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV he...
متن کاملDetection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.
Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which h...
متن کاملClinically relevant interpretation of solid phase assays for HLA antibody
PURPOSE OF REVIEW Accurate and timely detection and characterization of human leukocyte antigen (HLA) antibodies are critical for pre-transplant and post-transplant immunological risk assessment. Solid phase immunoassays have provided increased sensitivity and specificity, but test interpretation is not always straightforward. This review will discuss the result interpretation considering techn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Laboratory Medicine
سال: 2002
ISSN: 0007-5027,1943-7730
DOI: 10.1309/6ay1-hdf9-y09u-vvf2